LOCUS       sequence-112823-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant EGFP expression plasmid for the creation of a heteroduplex 
            DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see 
            notes in comments section..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Zhou B, Huang C, Yang J, Lu J, Dong Q, Sun LZ
  TITLE     Preparation of heteroduplex enhanced green fluorescent protein 
            plasmid for in vivo mismatch repair activity assay.
  JOURNAL   Anal Biochem. 2009 May 1;388(1):167-9. doi: 
            10.1016/j.ab.2009.02.020. Epub 2009 Feb 25.
  PUBMED    19248754
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.02.020"; journalName: "Anal Biochem"; date: 
            "2009-05-1- 1"; volume: "388"; issue: "1"; pages: "167-9"
COMMENT     Sequence Label: p189
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(19..38)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    48..129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     73..92
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(297..318)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     646..665
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccgcgggat gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
       61 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      121 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      181 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      241 tcgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      301 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      361 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      421 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      481 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      541 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      601 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      661 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      721 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
      781 gtggtcacga gggtgggcta gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      841 gtcagcttgc cgta
//
