LOCUS       sequence-112833-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of CCL20 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Affo S, Morales-Ibanez O, Rodrigo-Torres D, Altamirano J, Blaya D, 
            Dapito DH, Millan C, Coll M, Caviglia JM, Arroyo V, Caballeria J, 
            Schwabe RF, Gines P, Bataller R, Sancho-Bru P
  TITLE     CCL20 mediates lipopolysaccharide induced liver injury and is a 
            potential driver of inflammation and fibrosis in alcoholic 
            hepatitis.
  JOURNAL   Gut. 2014 Nov;63(11):1782-92. doi: 10.1136/gutjnl-2013-306098. Epub 
            2014 Jan 10.
  PUBMED    24415562
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1136/gutjnl-2013-306098"; journalName: "Gut"; date: "2014-11"; 
            volume: "63"; issue: "11"; pages: "1782-92"
COMMENT     Sequence Label: shCCL20
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..653
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(54..73)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     550..570
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
       61 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
      121 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
      181 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
      241 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
      301 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
      361 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      421 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      481 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      541 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      601 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
      661 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa
      721 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
      781 agctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      841 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
      901 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgt
//
