LOCUS       sequence-112836-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of NP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull
            X, Delgado-Garcia JM, Trullas R
  TITLE     Neuronal pentraxin 1 negatively regulates excitatory synapse density
            and synaptic plasticity.
  JOURNAL   J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 
            10.1523/JNEUROSCI.2548-14.2015.
  PUBMED    25855168
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2548-14"; journalName: "J Neurosci"; date: 
            "2015-04-8- 8"; volume: "35"; issue: "14"; pages: "5504-21"
COMMENT     Sequence Label: pLVTH-shNP1
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    58..175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      260..269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(266..268,269..271,272..982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(266..268,269..271,272..982)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(311..332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(572..591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     919..940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtgtcgtga ggaatttcga catttaaatt taattaatct cgacggtatc ggttaacttt
       61 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      121 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
      181 tcacgagact agcctcgagg tttaaactac gggatcttcg aaggcctaag cttacgcgcg
      241 cgtcctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      301 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      361 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      421 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      481 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      541 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      601 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      661 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      721 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      781 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      841 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      901 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      961 cggcatggac gagctgtaca agtccggact cagatctcga ctagctagta gctagctagc
     1021 t
//
