LOCUS       sequence-112837-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of NP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull
            X, Delgado-Garcia JM, Trullas R
  TITLE     Neuronal pentraxin 1 negatively regulates excitatory synapse density
            and synaptic plasticity.
  JOURNAL   J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 
            10.1523/JNEUROSCI.2548-14.2015.
  PUBMED    25855168
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2548-14"; journalName: "J Neurosci"; date: 
            "2015-04-8- 8"; volume: "35"; issue: "14"; pages: "5504-21"
COMMENT     Sequence Label: pLVTH-shNP1
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             6..186
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    206..239
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(325..539)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(385..404)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    join(568..586,587..609,610..628,629..651,652..670,671..693,
                     694..712,713..735,736..754,755..777,778..796,797..819,
                     820..838)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gatgctgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
       61 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
      121 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
      181 agacccagta caagcaaaaa gcagcataac ttcgtataat gtatgctata cgaagttatt
      241 cccatgcgac ggtatcgata agctaaaaag tacagccgcc tcaattcttc tcttgaaaga
      301 attgaggcgg ctgtacgggg atctgtggtc tcatacagaa cttataagat tcccaaatcc
      361 aaagacattt cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag
      421 ggcgccactc ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt
      481 tcccgcctag tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttcg
      541 aattcctgca gccccgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      601 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
      661 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
      721 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
      781 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
      841 ggtaaactcg agggggatcc actagcatgg tgaattgatc ccatcttgtc ttcgttggga
      901 gtgaattagc ccttccagtc cc
//
