LOCUS       sequence-112838-       11139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown of NP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull
            X, Delgado-Garcia JM, Trullas R
  TITLE     Neuronal pentraxin 1 negatively regulates excitatory synapse density
            and synaptic plasticity.
  JOURNAL   J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 
            10.1523/JNEUROSCI.2548-14.2015.
  PUBMED    25855168
REFERENCE   2  (bases 1 to 11139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2548-14"; journalName: "J Neurosci"; date: 
            "2015-04-8- 8"; volume: "35"; issue: "14"; pages: "5504-21"
COMMENT     Sequence Label: pLVTH-shNP1
FEATURES             Location/Qualifiers
     source          1..11139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2122..3299
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2353..3290
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3247..3267
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3347..3464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3549..3558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3555..3557,3558..3560,3561..4271)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3555..3557,3558..3560,3561..4271)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3600..3621)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3861..3880)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4208..4229
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4346..4934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4399..4419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4817..4828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5102..5120,5121..5143,5144..5162,5163..5185,5186..5204,
                     5205..5227,5228..5246,5247..5269,5270..5288,5289..5311,
                     5312..5330,5331..5353,5354..5372)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5401..5615
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5536..5555
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5701..5734)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5754..5934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5959..5977)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5960..5977)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6267..6286)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6500..6519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6619..6641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6679..6697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6765..6869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6870..6938,6939..7730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7088..7107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7901..8489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8390..8409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8643..8660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8730..8750)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8735..9064
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8915..9050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10198..10263
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10393..10413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10838..10972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10875..10894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10929..10948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct
     2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc
     2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
     3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
     3481 gcctcgaggt ttaaactacg ggatcttcga aggcctaagc ttacgcgcgc gtcctagcgc
     3541 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3601 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3661 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3721 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3781 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3841 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3901 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3961 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4021 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4081 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4141 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4201 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4261 agctgtacaa gtccggactc agatctcgac tagctagtag ctagctagct agtcgagctc
     4321 aagcttcggg gactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt
     4381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4741 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4861 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4921 ggccgcctcc ccgcatcggt acgtatggcc aggtaccttt aagaccaatg acttacaagg
     4981 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5041 cccaacgaag acaagatggg atcaattcac catgctagtg gatccccctc gagtttacca
     5101 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     5161 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     5221 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     5281 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     5341 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcggggct gcaggaattc
     5401 gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa
     5461 cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc
     5521 tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
     5581 gatttgggaa tcttataagt tctgtatgag accacagatc cccgtacagc cgcctcaatt
     5641 ctttcaagag aagaattgag gcggctgtac tttttagctt atcgataccg tcgcatggga
     5701 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc
     5761 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5821 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5881 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     5941 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     6001 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     6061 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     6121 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     6181 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     6241 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     6301 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     6361 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     6421 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     6481 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6601 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6661 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6721 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6961 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7021 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7081 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7201 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7261 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7321 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7381 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7441 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7681 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7741 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8041 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8281 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8641 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8701 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8761 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8881 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8941 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9001 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9061 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     9121 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     9181 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     9241 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     9301 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     9361 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     9421 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     9481 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9541 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9601 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9661 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9721 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9781 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9841 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9901 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9961 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
    10021 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
    10081 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
    10141 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    10201 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    10261 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    10321 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    10381 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    10441 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10501 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10561 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10621 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10681 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10741 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10801 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10981 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11041 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11101 gaaattgtat ttgttaaata tgtactacaa acttagtag
//
