LOCUS       sequence-112858-        3228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  phosphite dehydrogenase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Watanabe H, Hirakawa H,, Nagamune T.
  TITLE     Phosphite-driven Self-sufficient Cytochrome P450
  JOURNAL   ChemCatChem 2013 Dec;5(12):3835-3840
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ChemCatChem"; date: "2013-12"; volume: "5(12)"; pages: "3835-384"
COMMENT     Sequence Label: pHSG+PTDH
FEATURES             Location/Qualifiers
     source          1..3228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..192
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             193..852
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(259..278)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1060..1081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1096..1101,1102..1119,1120..1126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1134..1150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1139..1161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1158..1174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1158..1174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2245..2262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2245..2261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2254..2276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2416..2433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2587..3175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2667..2686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
       61 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      121 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      181 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      241 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      301 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      361 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      421 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      481 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      541 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      601 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      661 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      721 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
      781 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      841 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
      901 gaataagtga taataagcgg atgaatggca gaaattcagc ttggcccagt gccaagctcc
      961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattca tatgctgccg
     1201 aaactggtga tcacccaccg cgttcatgat gaaattctgc agctgctggc gccgcattgc
     1261 gaactgatga ccaaccagac cgatagcacc ctgacccgcg aagaaattct gcgccgttgc
     1321 cgcgatgcgc aggccatgat ggcgtttatg ccggatcgcg tggatgcgga ttttctgcag
     1381 gcgtgcccgg aactgcgtgt ggttggctgt gcgctgaaag gctttgataa ctttgatgtt
     1441 gatgcgtgta ccgcccgtgg tgtgtggctg acctttgtgc cggatctgct gaccgtgccg
     1501 accgcggaac tggcgattgg tctggcggtg ggcctgggtc gccatctgcg tgcggcggat
     1561 gcgtttgtgc gcagcggcga atttcagggc tggcagccgc agttttatgg caccggcctg
     1621 gataatgcga ccgttggcat tctgggtatg ggtgccattg gcctggcgat ggccgatcgc
     1681 ctgcagggct ggggcgccac cctgcagtat catgcgcgca aagcgctgga tacccagacc
     1741 gaacagcgcc tgggcctgcg tcaggtggcg tgtagcgaac tgtttgccag cagcgatttt
     1801 attctgctgg cgctgccgct gaacgcggat acccagcacc tggtgaatgc ggaactgctg
     1861 gcgctggttc gcccgggtgc cctgctggtt aatccgtgcc gtggcagcgt tgtggatgaa
     1921 gcggcggttc tggcggcgct ggaacgtggt cagctgggtg gttatgcggc cgatgtgttt
     1981 gaaatggaag attgggcgcg tgcggatcgc ccgcgcctga ttgatccggc gctgctggcc
     2041 cacccgaaca ccctgtttac cccgcatatt ggcagcgcgg tgcgcgcggt gcgtctggaa
     2101 atcgaacgct gcgccgcgca gaacattatt caggttctgg cgggtgcccg cccgattaat
     2161 gcggccaacc gtctgccgaa agcggaaccg gcggcgtgct gatgaggatc ctctagagtc
     2221 gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2281 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2341 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2401 agcttcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2761 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3001 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac tccgtcga
//
