LOCUS       sequence-112860-        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRABID NZF1-3 (aa 1-178) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINK-TRABID (NZF1-3, aa 1-178)
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(604..627)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(666..688)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gctctttggg ctnntagcag ccggatctcg tggtggtggt ggtggtgtta aatggtctag
       61 aaagctttaa ttaggtctgg gatgatcaca aacaacacat cttttagcct tggcccagtt
      121 ttcatatgtg caaacagagc aagtccagtg ctgtgtccta gtgttcagtt tatttctatc
      181 attgtattcc tcacaaggat caacagaaaa agcaactggt cttgagccag atcctgagga
      241 ctgaggagat tctgtaggac tcctggtcct acgttgggat aagcactggg tacatctgat
      301 tgctcttggc cagttcaaat atgtacacat gtggcatgac cacttatttg cattttccat
      361 gctatacgaa gatttcaccc ttggtcttgc actagagtct ggacatatca aaggactact
      421 tcctccttcg gtgctggaag gatcccaatc tctaccaaca tcacttgaac cacttttaaa
      481 tggatcttct gtaataattg ttccactagg tctttgggca cgacacatag tacacttgat
      541 tgcagatggc cagttttcat acgtacaata ttcacaagcc cacttaattc cacgttctga
      601 catcgggccc tgaaacagaa cttccagacc gctgctcaga tccgattttg gaggatggtc
      661 gccaccacca aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt
      721 caagtactta tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg
      781 gaacgcatcc aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat
      841 gaagtcagga tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc
      901 gaacattttc agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc
      961 tttactatat gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat
     1021 tgaaatctct gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata
     1081 acgtatgatg gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt
     1141 gggaaactcc aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgggctc
     1201 atacaaatgc tcttcatntt tttcttcann nnattccaaa nnaanncnaa nngggttgcc
     1261 aaaggccntt aattttccat aacctaanta aggggaanng gaagggnaag ggnatggncc
     1321 agggnnntcn cctcntaang taancaantn nttnnnangg ggaattntnn nccnnncaat
     1381 tcccntaaag nnngcnnnnt atttccggnn nnnnannncn nannnnna
//
