LOCUS       sequence-112861-        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRABID NZF1-3 (aa 1-178) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINK-TRABID (NZF1-3, aa 1-178)
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             608..625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(678..696)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      692..739
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      776..1231
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(863..882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1073..1094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1449..1462
                     /estimated_length=14
     gap             1520..1530
                     /estimated_length=11
     gap             1532..1543
                     /estimated_length=12
     gap             1579..1594
                     /estimated_length=16
     gap             1627..1637
                     /estimated_length=11
     gap             1652..1666
                     /estimated_length=15
     gap             1710..1731
                     /estimated_length=22
     gap             1770..1779
                     /estimated_length=10
     gap             1784..1869
                     /estimated_length=86
     gap             1877..1887
                     /estimated_length=11
ORIGIN
        1 gggnatcnca atnggannga gcacggtctg gaagttctgt tnngggcccg atgtcagaac
       61 gtggaattaa gtgggcttgt gaatattgta cgtatgaaaa ctggccatct gcaatcaagt
      121 gtactatgtg tcgtgcccaa agacctagtg gaacaattat tacagaagat ccatttaaaa
      181 gtggttcaag tgatgttggt agagattggg atccttccag caccgaagga ggaagtagtc
      241 ctttgatatg tccagactct agtgcaagac caagggtgaa atcttcgtat agcatggaaa
      301 atgcaaataa gtggtcatgc cacatgtgta catatttgaa ctggccaaga gcaatcagat
      361 gtacccagtg cttatcccaa cgtaggacca ggagtcctac agaatctcct cagtcctcag
      421 gatctggctc aagaccagtt gctttttctg ttgatccttg tgaggaatac aatgatagaa
      481 ataaactgaa cactaggaca cagcactgga cttgctctgt ttgcacatat gaaaactggg
      541 ccaaggctaa aagatgtgtt gtttgtgatc atcccagacc taattaaagc tttctagacc
      601 atttaaacac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      661 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      721 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      901 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      961 ttacggcacc tcgacccaaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1021 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1081 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1141 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1201 aattttaaca aantattaac gcttacaatt tagnnggnnc tttnccgggg aannnngnnc
     1261 ggaacccnta tttgttattt ttnnaaaaan ttcaannnng nntccgnnnn nnantnttnn
     1321 ntaaaaaann nntccnnncn naatggaact gganttnttn nnnccggnnn nanannnnnn
     1381 attttnnnnn acnnntnnnn ngnnangagg nnnaantnnc nnnngnnntc nnnaannnng
     1441 nnanncctnn nnnnnnnnnn nntnnanccc cnnnnaanaa annnnnntnn ttcccccnnn
     1501 aaaaannnnn nngnnnaacn nnnnnnnnnn gnnnnnnnnn nnnagnnnaa nnnnnnctnn
     1561 nnnnannntc agnnncccnn nnnnnnnnnn nnnnannnnn ncnnnntntn ngnnnnnnnn
     1621 nannaannnn nnnnnnnaan nnaannacnn cnnnnnnnnn nnnnnncnnn nnnngnannn
     1681 ngnnnnnnnn ggntnnnnnn nannaanaan nnnnnnnnnn nnnnnnnnnn nanacnaaaa
     1741 aaannannnn nnnnnaaann nnnnnnnaan nnnnnnnnna aaannnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnna aannaannnn nnnnnnnaaa aaaaaaaann nnnaannnnn nanaaaa
//
