LOCUS       sequence-112862-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-3xFLAG-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      142..329
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(197..221)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        404..747
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacggggga ggcgggggtg gagactacaa
       61 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
      121 gggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
      181 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
      241 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
      301 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag
      361 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
      421 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
      481 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
      541 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
      601 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
      661 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
      721 tcacatcaca tccgaacata aacaaccatg gcagaaccag cccaaaaaaa gcaaaaacaa
      781 actgttcagg agcgcaaggc gtttatctcc cgtatcacta atgaaa
//
