LOCUS       sequence-112863-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-3xFLAG-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(76..273)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(567..581)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaactggat ggcggcgtta
       61 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      121 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      181 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      241 gttcttgaaa acaagaatct ttttattgtc agtacttcac atcaaaacac ctttggttga
      301 gggaacgtca ttggtgccat tgctagaaat agcttctctt atggcaacag ccaaagcctt
      361 gaacgcactc tcacttctgt ggtgatcgtt gaaacctctc agacaatcaa catgcaaagt
      421 aattctggcc gcctccgcga aactttccaa aaagtgtgga atcatttcag tggataaatc
      481 accaatcatc tctctcttca atccaaggtc gattacagca aatggtctac tagataaatc
      541 gactacggca cgtgatagcg cctcatccaa tggtgcgaac ccagtaccga atctttttac
      601 accacggaca gcacccattg cttctttgaa cgcttgccct aatgcgatac cgcaatcttc
      661 ggtagtatgg tgatcgtcaa tgtgcaggtc accaatacat tcaacaataa gagaccaacc
      721 agagtgtttt gccaacgcat ggatcatatg atccaaaaag ccaacacctg tgtgaatatc
      781 gatgacctgt gactgagtag cttggg
//
