LOCUS       sequence-112864-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Chen ZJ, Ai HW
  TITLE     A highly responsive and selective fluorescent probe for imaging 
            physiological hydrogen sulfide.
  JOURNAL   Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. 
            Epub 2014 Sep 5.
  PUBMED    25141269
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi500830d";
            journalName: "Biochemistry"; date: "2014-09-23- 23"; volume: "53"; 
            issue: "37"; pages: "5966-74"
COMMENT     Sequence Label: pBAD-hsGFP
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     390..411
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(523..544)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgatgg tgatggtgcc agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
       61 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      121 ctcggcgcgg gtcttgtagg tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      181 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggga
      241 gaagcactgc acgccctagg tcaaggtggt cacgagggtg ggccagggca cgggcagctt
      301 gccggtggtg cagatgaact tcagggtcag cttgccgttg gtggcatcgc cctcgccttc
      361 gcccctcacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      421 caccccggtg aacagctcct cgcccttgct cactccggtg ccaccggatc cgccgtccac
      481 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      541 catgtgatcg cgcttctcgt tggggtcttt gctcagcacg gactgggtgc tcaggtagtg
      601 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      661 gagctgcacg ctgccgtcct ccacgttgtg gcggatcttg aagttcacct tgatgccgtt
      721 cttctgcttg tcggcggtga tatagacctt gtggctgttg tacgggctcg agcccatggt
      781 taattcctcc tgttagccca aaa
//
