LOCUS       sequence-112865-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-3xFLAG-kanMX6
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..125
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      149..336
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(204..228)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        411..754
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(822..841)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa tgggggaggc gggggtggag
       61 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
      121 acaagtaggg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      181 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      241 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      301 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt
      361 aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      421 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      481 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      541 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
      601 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      661 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      721 acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc
      781 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
      841 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
      901 gttgtttctg aa
//
