LOCUS       sequence-112868-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Chen ZJ, Ren W, Wright QE, Ai HW
  TITLE     Genetically encoded fluorescent probe for the selective detection of
            peroxynitrite.
  JOURNAL   J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. 
            Epub 2013 Sep 26.
  PUBMED    24059533
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja408011q";
            journalName: "J Am Chem Soc"; date: "2013-10-9- 9"; volume: "135"; 
            issue: "40"; pages: "14940-3"
COMMENT     Sequence Label: pcDNA3-pnGFP
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..101
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     499..519
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(588..605)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc
       61 agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac
      121 gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc
      181 cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca
      241 aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa
      301 aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg
      361 cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag
      421 gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc
      481 attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat
      541 ctttcacaaa ttttgtaatc cagaggttga ttgtcgacct cgagttaatg gtgatggtga
      601 tggtgcgtgt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      661 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      721 ttgtaggtgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      781 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggagaa gcactgcacg
      841 ccctaggtca aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      901 atgaacttca gggtcagctt gccgttgg
//
