LOCUS       sequence-112870-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 
            rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Smith RH, Levy JR, Kotin RM
  TITLE     A simplified baculovirus-AAV expression vector system coupled with 
            one-step affinity purification yields high-titer rAAV stocks from 
            insect cells.
  JOURNAL   Mol Ther. 2009 Nov;17(11):1888-96. doi: 10.1038/mt.2009.128. Epub 
            2009 Jun 16.
  PUBMED    19532142
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2009.128"; journalName: "Mol Ther"; date: "2009-11"; 
            volume: "17"; issue: "11"; pages: "1888-96"
COMMENT     Sequence Label: pSR657
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(162..173)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 catcagctgc tagcttacag attacgagtc aggtatctgg tgccaatggg gcgaggctct
       61 gaatacacgc cattagtgtc cacagtaaag tccacattaa cagacttgtt gtagttggaa
      121 gtgtactgaa tttcgggatt ccagcgtttg ctgttttcct tctgcagctc ccactcgatc
      181 tccacgctga cctgtcccgt ggagtactgt gtgatgaagg aagcaaactt tgccgcactg
      241 aaggtggtcg aaggattcgc aggtaccggg gtgttcttga tgagaatctg tggaggaggg
      301 tgtttaagtc cgaatccacc catgagggga gaggggtgaa aatgtccgtc cgtgtgtgga
      361 atctttgccc agatgggccc ctgaaggtac acatctctgt cctgccagac catgcctgga
      421 agaacgcctt gtgtgttgac atctgcggta gctgcttgtc tgttgcctct ctggaggttg
      481 gtagatacag aaccatactg ctccgtagcc acgggattgg ttgtcctgat ttcctcttcg
      541 tctgtaatca tgaccttttc aatgtccaca tttgttttct ctgagccttg cttcccaaag
      601 atgagaaccc cgctctgagg aaaaaacttt tcttcatcgt ccttgtggct tgccatggcc
      661 gggcccggat tcaccagaga gtctctgcca ttgaggtggt acttggtagc tccagtccac
      721 gagtattcac tgttgttgtt atccgcagat gtctttgata ctcgctgctg gcggtaacag
      781 ggtccaggaa gccagttcct agactggtcc cgaatgtcac tcgctccggc ctgagaaaac
      841 tgaagccttg actgcgtggt ggttccactt ggagtgtttg ttctgctcaa gtaatacagg
      901 tactggtcga tg
//
