LOCUS       sequence-112873-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-V5-hphMX4
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..90
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      114..301
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(169..193)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        376..719
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgggggag gcgggggtgg aaagcctatc
       61 cctaaccctc tcctcggtct cgattctacg tagggcgcgc cacttctaaa taagcgaatt
      121 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      181 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      241 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
      301 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg
      361 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      421 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
      481 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
      541 ctgcggacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
      601 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
      661 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
      721 tgggtaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
      781 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
      841 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
      901 atcgttatgt ttatcggc
//
