LOCUS       sequence-112883-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses only N terminus of RNF8, amino acids 1 to 140.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, 
            Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo 
            M
  TITLE     The scaffold protein WRAP53beta orchestrates the ubiquitin response 
            critical for DNA double-strand break repair.
  JOURNAL   Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114.
  PUBMED    25512560
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.246546"; journalName: "Genes Dev"; date: "2014-12-15- 
            15"; volume: "28"; issue: "24"; pages: "2726-38"
COMMENT     Sequence Label: p3xFLAG-CMV-RNF8-N-terminus(1-140)
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(632..697)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(727..778)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(756..776)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cccttgctcc ataccacccc cctccacccc ataatattat agaaggacac ctagtcagac
       61 aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt ggcaacttcc
      121 agggccagga gaggcactgg ggaggggtca cagggatgcc acccgggatc ctctagagtc
      181 gactggtacc ttaattcttt ggggaaagac aaggatatat tgtctcccag tcttcttcag
      241 taacttcata ttcatactcc gcattctcct tattttccag aggcactcca agttggatgt
      301 agtctccctg atgaatggaa tagaccctta aaggttccag acgcgctctg ttcagccaaa
      361 caccatttag actcttgttg tccataattg tccattggcc ctcaggattc tgcttcaaaa
      421 cacagtggtt tcgagaaatc atcagggggc agatttttga taccagttgg tatgtgacac
      481 caaatcctcg tcctacagtc acctcgcacc catcttccag cagcagccac ccggcgctca
      541 tccccacccg ccgcaggcac cagctccggc caccggcgcg gtctcctgtg acgaagaagc
      601 cgggctcccc cattgaattc gcggccgcaa gcttgtcatc gtcatccttg taatcgatgt
      661 catgatcttt ataatcaccg tcatggtctt tgtagtccat ggttaattct gacggttcac
      721 taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      781 cggggcgggg ttattacgac attttggaaa gtcccgttga ttt
//
