LOCUS       sequence-112884-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-eyfp
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  206..371
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     526..544
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    590..606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    623..639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             658..669
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgata agctctgata
       61 ccgctcgccg cagccgaacg accgagcgca gcgggtcagt gagcgaggaa gcggaagagc
      121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa
      181 cataacagga agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg
      241 tggaaatgga gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt
      301 gtagcgtcgt aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca
      361 cttatctggt tggcctgcaa ggtttatcag ggttattgtc tcatgagcgg atacatattt
      421 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      481 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      541 aggccctttc gtcttcacct cgagaaaatt tatcaaaaag agtgttgact tgtgagcgga
      601 taacaatgat acttagattc aattgtgagc ggataacaat ttcacacatc tagaattaaa
      661 gaggagaaat taagcatgct gagc
//
