LOCUS       sequence-112888-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 base vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  60..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(735..754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgcc caaacataac aggaagaaaa atgccccgct
       61 gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt aaggattatt
      121 tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta atacgaaaat
      181 taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct gcaaggcctt
      241 cgcgaggtac cgggcccaag cttctcgagg aattcctgca gcccggggga tccactagtg
      301 agctcatgca tgatatcgtc gaccccacgc ccctctttaa tacgacgggc aatttgcact
      361 tcagaaaatg aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat
      421 aactggactg atttcagttt acaactattc tgtctagttt aagactttat tgtcatagtt
      481 tagatctatt ttgttcagtt taagacttta ttgtccgccc acacccgctt acgcagggca
      541 tccatttatt actcaaccgt aaccgatttt gccaggttac gcggctggtc ctctagctgg
      601 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      661 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      721 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccag
//
