LOCUS       sequence-112891-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of TpR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFTP1
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..80)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(132..137,138..154,155..160)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 taccgagctc gaattagctt caaaagcgct ctgaagttcc tatactttct agagaatagg
       61 aacttcggaa taggaacttc aagatcccct gattcccttt gtcaacagca atggataatt
      121 cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc
      181 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      241 ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
      301 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      361 agggcagtcg ccctaaaaca aagttaggca gccgttgtgc tggtgctttc tgatagttgt
      421 tgtggggtag gcagtcagag ctcgatttgc ttgtcgccat aatagattca caagaaggat
      481 tcgacatggg tcaaagtagc gatgaagcca acgctcccgt tgcagggcag tttgcgcttc
      541 ccctgagtgc cacctttggc ttaggggatc gcgtacgcaa gaaatctggt gccgcttggc
      601 agggtcaagt cgtcggttgg tattgcacaa aactcactcc tgaaggctat gcggtcgagt
      661 ccgaatccca cccaggctca gtgc
//
