LOCUS       sequence-112894-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 base vector with transcriptional terminator and oriT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18T-mini-Tn7T
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  60..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(345..431)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(534..628)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(656..672)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgcc caaacataac aggaagaaaa atgccccgct
       61 gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt aaggattatt
      121 tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta atacgaaaat
      181 taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct gcaaggcctt
      241 cgcgaggtac cgggcccaag cttctcgagg aattcctgca gcccggggga tccactagtg
      301 agctcatgca tgatatcgtc cgggccgcaa gctcctagcg gcggatttgt cctactcagg
      361 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
      421 cgttttattt gatgcctcaa gctagagagt cattacccca ggcgtttaag ggcaccaata
      481 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtctagct tggattctca
      541 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      601 gtcattactg gatctatcaa caggagtcca agctcagcta attaagctag cttatcgata
      661 ccgtcgacct cgaaccccac gcccctcttt aatacgacgg gcaatttgca cttcag
//
