LOCUS       sequence-112907-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-GW
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  60..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    256..380
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(405..410,411..428,429..435)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(555..574)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagcgcc caaacataac aggaagaaaa atgccccgct
       61 gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt aaggattatt
      121 tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta atacgaaaat
      181 taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct gcaaggcctt
      241 cgcgaggtac ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
      301 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      361 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
      421 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
      481 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      541 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      601 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      661 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
      721 aagacggtga gctggtgata tgggatagtg
//
