LOCUS       sequence-112910-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 base vector with transcriptional terminator and oriT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18R6KT-mini-Tn7T-Km
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    343..390
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     mobile_element  505..670
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
       61 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
      121 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
      181 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
      241 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccatgca tttgttaaat
      301 cgaattatcc attgctgttg acaaagggaa tcaggggatc ttgaagttcc tattccgaag
      361 ttcctattct ctagaaagta taggaacttc agagcgcttt tgaagctaat tcgagatcat
      421 gcatgagctc actagtggat cccccgggct gcaggaattc ctcgagaagc ttgggcccgg
      481 tacctcgcga aggccttgca ggccaaccag ataagtgaaa tctagttcca aactattttg
      541 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
      601 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
      661 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgca
//
