LOCUS       sequence-112912-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 base vector with transcriptional terminator and oriT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18R6KT-mini-Tn7T-Km
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(169..216)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(264..350)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(453..547)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(575..591)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(576..592)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
       61 aattgaaaaa ggaagagtat gaggatccaa catttccaat cactagtgaa ttatctagaa
      121 ttattccatt gagtaagttt ttaagcacat cagcttcaaa agcgctctga agttcctata
      181 ctttctagag aataggaact tcggaatagg tacttcaaga tccccaattc gagatcgtcc
      241 gggccgcaag ctcctagcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      301 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctcaag
      361 ctagagagtc attaccccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
      421 cccgccctgc cactcatcgc agtctagctt ggattctcac caataaaaaa cgcccggcgg
      481 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      541 aggagtccaa gctcagctaa ttaagctagc ttatcgatac cgtcgacctc gaaccccacg
      601 cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat
      661 aacaaaagtc cagtatgctt tttcacagca taactggact gatttcag
//
