LOCUS       sequence-112914-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 3. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ30
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(185..190,191..207,208..213))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    254..301
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(385..407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
       61 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
      121 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
      181 ttacagttta cgaaccgaac aggcttatgt caattcgatc cattgctgtt gacaaaggga
      241 atcaggggat cttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
      301 cagagcgctt ttgaagctaa ttcgatcatg catgagctcg aattcccggg gatccgtcga
      361 cctgcagcca agcttcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      421 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      481 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
      541 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
      601 cgatactgtc gtcgt
//
