LOCUS       sequence-112934-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CREB1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CREB1_1
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(283..336)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(352..399)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
       61 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      121 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      181 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      241 gatgggcacc accccggtga acagctcctc gcccttgctc actgggccag gattctcctc
      301 gacgtcaccg catgttagca gacttcctct gccctctcca ctgccgaatt cctttttctt
      361 ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca gctgagacag
      421 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      481 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      541 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      601 gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag
      661 gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      721 ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata
      781 gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt
      841 ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga
      901 gtacttaggc agcttgatga tcaggtcctt tt
//
