LOCUS       sequence-112935-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector with Ptac expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T-LAC
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..150)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(191..196,197..213,214..219)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
       61 gcgcaacgca atgcatgatc gaattagctt caaaagcgct ctgaagttcc tatactttct
      121 agagaatagg aacttcggaa taggaacttc aagatcccct gattcccttt gtcaacagca
      181 atggatcgaa ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
      241 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
      301 tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg
      481 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg
      541 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg
      601 attacctcgg gaac
//
