LOCUS       sequence-112936-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector with Ptac expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18-mini-Tn7T-LAC
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(297..302,303..318,319..325)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    333..349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     338..360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     mobile_element  452..617
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga
       61 tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat tgcaagctga tccgggctta
      121 tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg
      181 ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga
      241 taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga
      301 caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg
      361 aaacagaatt cgagctcact agtggatccc ccgggctgca ggaattcctc gagaagcttg
      421 ggcccggtac ctcgcgaagg ccttgcaggc caaccagata agtgaaatct agttccaaac
      481 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
      541 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
      601 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttgggcg ctgcattaat
      661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      721 tcactgaccc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactc
//
