LOCUS       sequence-112937-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector with oriR6K, for attTn7 site determination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm-REP
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      221..577
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gggcggacaa aatagttggg aactgggagg ggtggaaatg gagtttttaa ggattattta
       61 gggaagagtg acaaaataga tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta
      121 aaaatgacaa aatagtttgg aactagattt cacttatctg gttggcctgc aaggatctgc
      181 agaattcggc ttattaccgc ggcagttcaa cctgttgata gtacgtacta agctctcatg
      241 tttcacgtac taagctctca agtttaacgt actaagctct catgtttaac gaactaaacc
      301 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
      361 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
      421 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
      481 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
      541 caattttgag tgacacagga acacttaacg gctgacaacc ggttaataag ccgaattcca
      601 gcacactggc ggccgttact agtggatcca ctagtgagct catgcatgat c
//
