LOCUS       sequence-112943-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human ATF1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_ATF1_1
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        102..350
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     102..122
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     273..292
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        372..447
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
       61 tttacggttc ctggcctttt gctggccttt tgctcacatg tgagggccta tttcccatga
      121 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
      181 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      241 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      301 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gtaggaatca
      361 aacactttta tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      421 cttgaaaaag tggcaccgag tcggtgcttt tttgttttag agctagaaat agcaagttaa
      481 aataaggcta gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt
      541 acccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      601 attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      661 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      721 tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga
      781 ctttcctact tg
//
