LOCUS       sequence-112946-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 2. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ20
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  49..214
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gattcattaa tgcagcgccc aaacataaca ggaagaaaaa tgccccgctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttggcctg caaggaaata gtacataatg
      241 gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta tttttgacac
      301 cagaccaact ggtaatggta gcgaccggcg ctcagctgta attccgccga tactgacggg
      361 ctccaggagt cgtcgccacc aatccccata tggaaaccgt cgatattcag ccatgtgcct
      421 tcttccgcgt gcagcagatg gcgatggctg gtttccatca gttgctgttg actgtagcgg
      481 ctgatgttga actggaagtc gccgcgccac tggtgtgggc cataattcaa ttcgcgcgtc
      541 ccgcagcgca gaccgttttc gctcgggaag acgtacggga tatacatgtc tgacaatggc
      601 agatcccagc ggtcaaaaca ggcggcagta aggcggtcgg gatagttttc ttgcggccct
      661 aatccgagcc agtttacccg ctctgctacc tgcgccagct ggcagttcag gccaatccgc
      721 gccggatgcg gtgtatcgct cgccacttca acatcaacgg taatcgccat ttgaccacta
      781 ccatcaatcc ggtagg
//
