LOCUS       sequence-112950-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human RAD21 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_RAD21_1
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(289..342)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(358..405)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcactg ggccaggatt
      301 ctcctcgacg tcaccgcatg ttagcagact tcctctgccc tctccactgc cgaattcctt
      361 tttctttttt gcctggccgg cctttttcgt ggccgccggc cttttgtcgc ctcccagctg
      421 agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc
      481 cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa
      541 ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc
      601 tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc
      661 ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg
      721 cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt
      781 ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt
      841 tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa
      901 cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc
      961 ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag
     1021 cagctctttc acactcttca gtttcttgga cttgcccttt t
//
