LOCUS       sequence-112955-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, for construction of B-galactosidase protein 
            fusions, frame 1. See Depositor Comments below for information 
            regarding the lacZ sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18-mini-Tn7T-Gm-LacZ10
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..53
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(138..160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
       61 ttttgaagct aattcgatca tgcatgagct cgaattcccg gggatccgtc gacctgcagc
      121 caagcttgcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
      301 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
      361 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg
      421 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
      481 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
      541 gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag
      601 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
      661 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg
      721 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
      781 gatttccatg ttg
//
