LOCUS       sequence-112958-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of GmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFGM1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..72
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(133..138,139..155,156..161)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 taccgagctc gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag
       61 tataggaact tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaataa
      121 ttctagatcg aattgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
      181 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      241 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
      301 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      361 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
      421 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
      481 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
      541 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
      601 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
      661 tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac
      721 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
      781 gcaagcagat tac
//
