LOCUS       sequence-112960-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of CmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFCM1
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..72
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        157..259
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(326..345)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taccgagctc gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag
       61 tataggaact tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaataa
      121 ttctagatgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      181 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      241 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      301 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      361 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      421 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggagttccg
      481 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      541 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      601 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      661 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      721 cagttttgat ttaaacgtgg ccaatatgga caacttcttc g
//
