LOCUS       sequence-112967-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses siRNA resistant WRAP53beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, 
            Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo 
            M
  TITLE     The scaffold protein WRAP53beta orchestrates the ubiquitin response 
            critical for DNA double-strand break repair.
  JOURNAL   Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114.
  PUBMED    25512560
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.246546"; journalName: "Genes Dev"; date: "2014-12-15- 
            15"; volume: "28"; issue: "24"; pages: "2726-38"
COMMENT     Sequence Label: pCMV-FLAG-WRAP53beta-siRNA-resistant
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccgcca ccatggatta caaggatgac gacgataaga gcccgggcgg atcccccggg
       61 ctgcaggaat tcatgaagac tttggagact caaccgttag ctccggactg ctgtccttca
      121 gaccaggacc cagctccagc ccatccttct ccccacgctt ccccgatgaa taaaaatgcg
      181 gactctgaac tgatgccacc gcctcccgaa aggggggatc cgccccggtt gtccccagat
      241 cctgtggctg gctcagctgt gtcccaggag ctacgggagg gggacccagt ttctctctcc
      301 actcccctgg aaacagagtt tggttcccct agtgagttga gtcctcgaat cgaggagcaa
      361 gaactttctg aaaatacaag ccttcctgca gaagaagcaa acgggagtct ctctgaagaa
      421 gaagcgaacg ggccagagtt ggggtctgga aaagccatgg aagatacctc tggggaaccc
      481 gctgcagagg acgagggaga caccgcttgg aactacagct tctcccagct gcctcgattt
      541 ctcagtggtt cctggtcaga gttcagcacc caacctgaga acttcttgaa aggctgtaag
      601 tgggctcctg acggttcctg catcttgacc aatagtgctg ataacatctt gcgaatttat
      661 aacctgcccc cagagctgta ccatgagggg gagcaggtgg aatatgcaga aatggtccct
      721 gtccttcgaa tggtggaagg tgataccatc tatgattact gctggtattc tctgatgtcc
      781 tcagcccagc cagacacctc ctacgtggcc agcagcagcc gggagaaccc gattcatatc
      841 tgggacgcat tcactggaga gctccgggct tcctttcgcg cctacaacca cctggatgag
      901 ctgacggcag cccattcgct ctgcttctcc c
//
