LOCUS       sequence-112968-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCH_ATF1
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccacc ggaacctcct ccgctcccca
      241 cggacttgtt ggaataaaga tccttcaaag tttttaactc ttctattaga gttttatttt
      301 gattttccag gactgcaact cggttttcca ggcatttcac atattctttc ttctttctgc
      361 gacattctcg agcagcttct ctaaatatga gcattaaaaa aacaatgtta gaaaaaactt
      421 cacattcttt aaatggtaaa tgttcttcag taattttccg tggtcacctt tttttctctt
      481 taaagtatca tctacacaca gagaatacat agatcttaag tgtagaattc agtcagtttt
      541 ggcaattgtg tatacctgta taacccacac ctctgtcaag acaacagaac attcccactg
      601 tcccagaaag ttccctcatg ccccttcctg gtcagccccc ttccaagcaa ctatcgtttt
      661 gatttttatc accagagatt agtttttttc ctattctaga acctcatgta agagtgtcat
      721 acactatgta ctctggtgtc ccttcactca gcccatctga ggttcatcca tgttgctgca
      781 tgggggtttg ttccttcaca ctgctggaca tagttcactg tatgaatata accaccattt
      841 gtgtatccag tctcttgcga tgtttctagg gttagctgca taggcatgtt tttgtgccag
      901 tctttttgta gaaaggtttc atttctcttg aataaatacc aaagagtgaa attatgggtc
      961 ctctggttaa cttctgacac atgacttctt t
//
