LOCUS       sequence-112970-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human 
            GABPA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_GABPA
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccacc ggaacctcct ccgctcccat
      241 tatccttttc cgtttgcaga gaagcagtag ccagagctac tgctgtgact ggctgggcaa
      301 ttccatggag ctgcatcttt gcaagtttct tctgttcaca ttctgtgacc aaacggttca
      361 actccgctgc actgtatcca ataagagtct tcaagtcaca gacaaacttg tacacaaatc
      421 tcttgccttg aactttacaa atcatgtccc catcgtaata atatcttcaa aaagagaaaa
      481 aaaaattaga gacttcagaa aaaataaatg agacttctta caataattta cagactattc
      541 cataagcaga agcctagtag agtactgcat ccgccacaat agcaattaat attgaagcta
      601 tgtagaatat ttaatgctac tatcaaatga aacatcccaa cattatttac taaaatttca
      661 atgctcacta ctccaaaact ctatacatta aactttcaaa acatattttc aacaattaga
      721 ataaaacatt gatttaaaaa taattattaa acaaaacaaa aaaccttgag agcagtagaa
      781 gagcctaaga acaagagctt tggagtcaga aagccaagat tcaaaaccta acatttttga
      841 gaccacagga gtcatatttt ctcaaa
//
