LOCUS       sequence-112972-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human 
            CREB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCH_CREB1
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(720..737)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(720..736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..751)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgat tcgaacatcg ggatttaaat tttcacctgt
      121 taaggtggaa aatggactgg cttggccaca acctgaaaga caaaataaac attttatttt
      181 ctaaacattt ctttttttct atgcgcaaaa ctgcctgaaa gcaactacag aatttcattc
      241 atttgtgctt ttgcattaaa ctgtgaatgt tccaacacct gcctccactt ctcccctcaa
      301 gaaattttca acgccaggaa tcatgaagag acttctgctt ttcaaccccc accctcctca
      361 agaagtaata atttgtttac ttgtaaattg atgggagaaa tgaggaaaag aaaatctttt
      421 taaaaatgat ttcaaggttt gtgctgagct ccttgattgc cttagggaca gaattacccc
      481 agcctcttga gctgaagtaa tgtgtgggcc gcatgcataa agtaagtaag gtgcaatgaa
      541 gaagtgttga ttgccaaatt gacatgttgt cacattctca ttgtgaatta tgtaaagttg
      601 ttaagagaca taccctctaa aaaagaactt tagcatggta ttgaaggaat tagaaatgaa
      661 tttggagtgc tttttatgtc ttggaaagtc ctctccactg actgtagcct ccaaaattca
      721 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      781 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      841 ccttcccaac agttgcgcag cctgaatggc gaatgcgatt tattcaacaa agccgccgtc
      901 ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa
      961 aa
//
