LOCUS       sequence-112975-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human 
            CREB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCH_CREB1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..108)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(124..147)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(151..174)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(178..201)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc caggtccagg
       61 gttctcctcc acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttcctga
      121 aaccttatcg tcgtcatcct tgtaatctcc cttatcgtcg tcatccttgt aatcgccctt
      181 atcgtcgtca tccttgtaat ctccagaacc acctccaccg gaacctcctc cgctcccatc
      241 tgatttgtgg cagtaaaggt ccttaagtgc ttttagctcc tcaatcaatg tcttgttttg
      301 attttcaagc actgccactc tgttttctaa acatttcaca tattctttct tctttctacg
      361 acactctcga gctgcttccc tacaagcaaa agaggacggg aaaaattatg caaatgattt
      421 ccacatattt attttggatt accttttttt tttctttttt aaagaaatag ctcacagcaa
      481 ccaacatttt agtatatatt aagcattgga aaagatatac agaattactt ggaaattacc
      541 ttatattaaa attttaatct ggccaggcgt ggcggctcac gcctgtaatc ccagcacttt
      601 cgggggccaa ggcagggaga tcacctgagg tcaggagtcc aagatcagcc tggccaacat
      661 gatgaaaccc ctctctacta taaacacaaa aattagcggg gtgtggcggc acatgcctgt
      721 agtcccagct acttggaagg ctgagccatg ggcatcgctt gaacctggga ggcagaggct
      781 gcagtaagct gagattgcgc cactgcactc cagcctgagt gacaagagtg agactccgtc
      841 tcaaagaaac aaacaaaaaa acaactccat aaacaaacaa gacctaaatg accacttaaa
      901 aa
//
