LOCUS       sequence-112982-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human 
            RAD21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_RAD21
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..105)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(121..144)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(148..171)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(175..198)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatccag gtccagggtt
       61 ctcctccacg tctccagcct gcttcagcag gctgaagtta gtagctccgc ttcctgaaac
      121 cttatcgtcg tcatccttgt aatctccctt atcgtcgtca tccttgtaat cgcccttatc
      181 gtcgtcatcc ttgtaatctc cagaaccacc tccaccggaa cctcctccgc tccctataat
      241 atggaacctt ggtccaggtg ttgcgatgat gtcactgtac ggttcttcct gtgtcagctc
      301 aatagcttgc tgctttttaa gaaccaagaa gctgtagaac tttgcggcag cttgttttct
      361 gttcgtattt cgacataact caagcaaact gatagattca gctccagttt tagcaagagc
      421 acgctgaaat aaaaccaaaa aagggtaact taatctgtat aataaagtag taattcagtg
      481 aggatgctga tacagtttga atatttgtcc cctccaaatc tcatgctgaa acgtaatttc
      541 ccagtgttag agatggagcc tagtgaggtg tttgtgtcat ggggtgggga tgggggtggt
      601 ggtggatccc ttatcaatca ctccctgtgg caatcagtga attcctgctc gttagttaac
      661 acaagagctg cttgtttaaa agcgcctagc catctctctc atgccctctc ttgccatgtg
      721 acacacctgc tccccctttg ccttccacca tgactataag ctttctgagg cctcaccaga
      781 agcagatgct ggtgctttgc ttcttgtata gtctgcagaa ctgtgagcca aataaacttc
      841 tttataaatt acccagactc aggtattcct ttagagcaac acaaaacaga ctaacacaga
      901 tgctaatttt
//
