LOCUS       sequence-112984-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3X Flag with P2A-Neo for 3' tagging of endogenous proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_Donor (EMM0021)
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc cataggagac accgggaccg atccagcctg gggatcccga ccatggcttc
      121 gtacccctgc catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag
      181 caaccgacgt acggcgttgc gccctcgccg gcagcaagaa gccacggaag tccgcctgga
      241 gcagaaaatg cccacgctac tgcgggttta tatagacggt cctcacggga tggggaaaac
      301 caccaccacg caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga
      361 gccgatgact tactggcagg tgctgggggc ttccgagaca atcgcgaaca tctacaccac
      421 acaacaccgc ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag
      481 cgcccagata acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat
      541 cgggggggag gctgggagct cacatgcccc gcccccggcc ctcaccctca tcttcgaccg
      601 ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga taccttatgg gcagcatgac
      661 cccccaggcc gtgctggcgt tcgtggccct catcccgccg accttgcccg gcacaaacat
      721 cgtgttgggg gcccttccgg aggacagaca catcgaccgc ctggccaaac gccagcgccc
      781 cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc
      841 caatacggtg cggtatctgc agggcggcgg gtcgtggcgg gaggattggg gacagctttc
      901 ggggacggcc gtgccgcccc agggtgccga gccccagagc aacgcgggcc cacgacccca
      961 tatcggggac acgttattta ccctgtttcg ggccccc
//
