LOCUS       sequence-112991-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full length RCAN1 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             340..363
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgatatgg actacaaaga cgatgacgac
      361 aagcgaattc cccggggatc catggaggag gtggatctgc aggacctgcc gagcgccacc
      421 atcgcctgcc acctggaccc gcgcgtgttc gtggacggcc tgtgccgggc caaatttgaa
      481 tccctcttca gaacatatga caaggacacc accttccagt attttaagag cttcaaacgt
      541 gtccggataa acttcagcaa ccccttatct gcagccgatg ccaggctgcg gctgcacaag
      601 accgagttcc tggggaagga aatgaagttg tattttgctc agactttaca cataggaagt
      661 tcacacctgg ctccgcccaa tcccgacaaa cagttcctca tctcccctcc ggcctctcct
      721 cccgttggct ggaaacaagt agaagatgcc accc
//
