LOCUS       sequence-112998-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT010
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      155..342
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(210..234)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(461..658)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actcactata gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg
       61 gatctataac ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg
      121 ttcctgacta tgcgactagt taacttctaa ataagcgaat ttcttatgat ttatgatttt
      181 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
      241 tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca
      301 ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa
      361 cagggtaagg atccagattg tactgagagt gcaccataat catcgatgaa ttcgagctcg
      421 ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc
      481 acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga
      541 tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa
      601 tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac
      661 tgattattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
      721 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
      781 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
      841 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
      901 ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
      961 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
     1021 cccgaacatc gcctcgctcc agtca
//
