LOCUS       sequence-113000-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT010
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..30
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(66..113)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(122..155)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        250..593
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc ttgcatgcgc ggccgcttat
       61 gtacacttgt catcgtcatc tttataatct ttatcgtcat cgtccttgta gtcgtcgact
      121 gataacttcg tatagcatac attatacgaa gttattctag actgtgcggt atttcacacc
      181 gcatagggtt aattaaggcg cgccagatct gtttagcttg cctcgtcccc gccgggtcac
      241 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
      301 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
      361 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg
      421 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
      481 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
      541 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
      601 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
      661 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
      721 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
      781 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
      841 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
      901 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
      961 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaa
//
