LOCUS       sequence-113004-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pTW073
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..100
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      156..343
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(211..235)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(462..659)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gactcactat agggcgaatt gggcccaatg cattggcgcc gcggccgcgg tggatctggt
       61 ggatctataa cttcgtataa tgtatgctat acgaagttat caggtaccta cccatacgat
      121 gttcctgact atgcgactag ttaacttcta aataagcgaa tttcttatga tttatgattt
      181 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      241 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      301 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
      361 acagggtaag gatccgcatt gtactgagag tgcaccataa tcatcgatga attcgagctc
      421 gttttcgaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt
      481 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
      541 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
      601 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
      661 ctgattattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
      721 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
      781 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
      841 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
      901 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
      961 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     1021 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tcc
//
