LOCUS       sequence-113009-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
  TITLE     Cerulean, Venus, and VenusY67C FRET reference standards.
  JOURNAL   Biophys J. 2006 Dec 15. 91(12):L99-L101.
  PUBMED    17040988
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2006 Dec 15. 91(12):L99-L101."
COMMENT     Sequence Label: mCerulean C1
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    124..245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(135..154)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     189..208
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    369..434
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(435..686)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(477..498)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     826..845
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
       61 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg
      121 cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      181 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      241 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      301 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      361 gatccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga
      421 gatctgagtc cggacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      481 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg
      541 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      601 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg
      661 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatggcgttg
      721 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      781 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      841 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaa
//
