LOCUS       sequence-113010-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pTW073
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    39..95
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    complement(160..193)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        288..631
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 acgccaagct cggaattaac cctcactaaa gggaacaaaa gcttgcatgc ctgcaggtcg
       61 actctagagg atccccgggt accgagctcg aattcgcggc cgcttatgta caagaatcga
      121 gaccgaggag agggttaggg ataggcttac cgtcgactga taacttcgta tagcatacat
      181 tatacgaagt tattctagac tgtgcggtat ttcacaccgc atagggttaa ttaaggcgcg
      241 ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
      301 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
      361 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
      421 gcacggcgcg aagcaaaaat tacggctcct cgctgcggac ctgcgagcag ggaaacgctc
      481 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
      541 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
      601 gttctcacat cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac
      661 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      721 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      781 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
      841 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
      901 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
      961 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atct
//
