LOCUS       sequence-113012-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of EPHB3 and GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-EphB3-SV40-GFP (B3G)
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(26..236)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      32..167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(37..334)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        184..375
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     332..352
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gctcaccatg gtggctagct gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
       61 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
      121 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
      181 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
      241 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
      301 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
      361 ttctttccgc ctcagaaggt acctaaccaa gttcctcttt cagaggttat ttcaggccat
      421 ggtgctgcgc accggttcag acctgcacag gcagcgtctg gttcatctgc agccgcatgt
      481 cctggatact gctcaggatc ttcttctggt ggccggccag ggtgacccca atacggagca
      541 ggtcttctgc cgtcatctgg gccaccaggt caaaagatgc aaaccccgca ctgacgaagc
      601 tctccttgta ccgccccatc ttgatggcat ccagccaatc accaactgtc gtgaaggttg
      661 tgtaatctgg gaccgtgcgg tccaggaggg gctgtgacat gccagactga gcgctggcaa
      721 tgaccttgag gctggcagca ttgcggatga gcttgtccag ggtattgaca atctgg
//
