LOCUS       sequence-113014-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP 
            tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pVM013
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             108..140
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      161..348
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(216..240)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(467..664)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actcactata gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg
       61 gatctataac ttcgtataat gtatgctata cgaagttatc aggtaccatg gcaagcatga
      121 ctggtggaca gcaaatgggt actagttaac ttctaaataa gcgaatttct tatgatttat
      181 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      241 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      301 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      361 ggataacagg gtaaggatcc gcattgtact gagagtgcac cataatcatc gatgaattcg
      421 agctcgtttt cgacactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca
      481 gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc
      541 agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa
      601 ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt
      661 cagtactgat tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
      721 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
      781 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
      841 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
      901 atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga a
//
