LOCUS       sequence-113016-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP 
            tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pVM013
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..37)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(46..79)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        174..517
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 acattgtaca cgcatagtca ggaacatcgt atgggtagtc gactgataac ttcgtatagc
       61 atacattata cgaagttatt ctagactgtg cggtatttca caccgcatag ggttaattaa
      121 ggcgcgccag atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg
      181 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact
      241 gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg
      301 atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc gagcagggaa
      361 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa
      421 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag
      481 gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaaaagc ctgaactcac
      541 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
      601 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
      661 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
      721 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
      781 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
      841 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
      901 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
      961 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     1021 cgacaccgtc agtgcgtccg tc
//
